Elementolab/ChIPseeqer Species

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Other programs in the ChIPseeqer framework that you need to specify Other programs in the ChIPseeqer framework that you need to specify
- * the genome (e.g., -genome "hg18") and/or+ * the genome (e.g., --genome=hg18) or
 + * the database (e.g., --db=AceView) or
* the gene annotation files (e.g., annotation=DATA/mm9/refGene.txt.mm9.20APR2010) * the gene annotation files (e.g., annotation=DATA/mm9/refGene.txt.mm9.20APR2010)
are: are:

Revision as of 20:17, 19 April 2011

Elementolab/

SUPPORTED SPECIES

The species currenty supported in ChIPseeqer are:

  • Human (acembly hg18) - Default in all ChIPseeqer programs
  • Mus musculus (acembly mm9)
  • Drosophila melanogaster (acembly dm3)
  • Saccharomyces cerevisiae

To run ChIPseeqer on a species make sure to use the option:

-chrdata STR     to run for organisms other that human (default is hg18), point to files:
                 DATA/mm9.chrdata for mouse, 
                 DATA/dm3.chrdata for drosophila or 
                 DATA/sacser.chrdata for Saccharomyces cerevisiae

Other programs in the ChIPseeqer framework that you need to specify

* the genome (e.g., --genome=hg18) or
* the database (e.g., --db=AceView) or
* the gene annotation files (e.g., annotation=DATA/mm9/refGene.txt.mm9.20APR2010)

are:

ChIPseeqerAnnotate
ChIPseeqerSummaryPromoters
ChIPseeqerFindClosestGenes
ChIPseeqerFindDistalPeaks
ChIPseeqerDensityMatrix
ChIPseeqerPlotAverageReadDensityInGenes
ChIPseeqerPlotAveragePeaksNumberInGenes
ChIPseeqerCreateRandomRegions

HOW TO ADD A NEW SPECIES

1. You need to make a .chrdata file for the new organism, that will look like this:

chr10	129993255	0.840
chr11	121843856	0.866
chr12	121257530	0.797
chr13	120284312	0.816
chr14	125194864	0.781
chr15	103494974	0.842
chr16	98319150	0.838
chr17	95272651	0.812
chr18	90772031	0.837
chr19	61342430	0.830

and the columns indicate (chromosome_name chromosome_size chromosome_mappability)

To create this file follow the steps here.

2. Add the .chrdata to the ChIPseeqer/DATA/ folder

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