TBAG Tutorial

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MasonLab

TBAG

Installation Instruction

BAGET Tutorial

• Preprocessing: Preprocessing the .fastq data using bwa alignment

• Genome BAGET: Annotation for RNA-seq reads which have been aligned to genome such as hg18

• Junction BAGET: Annotation for RNA-seq reads which have been aligned to junction genome such as Aceview.fa

• PolyA BAGET: Annotation for RNA-seq reads with polyA-tail which have been truncated and the non-tail been aligned to genome such hg18
  • PAS: Poly-adenylation cleavage site distribution analysis of polytbag output
  • MIR: 3'UTR miRNA predicted targeting site analysis of polytbag output

• Fusion BAGET: Annotation for RNA-seq reads which is fusion protein and have been aligned to genome such hg18