Phosphorylation BioWizard

SigPath's BioWizards are user interfaces designed to feel user-friendly to Biologists. The phosphorylation BioWizard allow users to submit phosphorylation modifications. The following types of phosphorylations are currently supported:

Protein phosphorylations.

Small molecule phosphorylations.

Phosphorylations of complexes of any of the above.

The phosphorylation BioWizard guides the user through the selection of the substrate of the reaction. The user is then prompted to enter the enzyme (which must be a protein or protein-containing complex). The user can then enter known interaction sites on the substrate or enzyme, and the publications that describe the phosphorylation. Kinetic parameters such as Vmax (maximum velocity of the reaction), Km (Michaelis-Menten constant), and KbKp (ratio of the rates of E.S -> E+S and E.S -> E+P) can be entered to allows the enzymatic reaction to be included in quantitative models. Some of these parameters are optional and can be entered at a latter time, potentially by a different user than the original submitter for the interaction.

The current prototype of SigPath has limited support for the reviewing of the information submitted, so that submissions to the information system must be co-ordinated with the members of the project.

The phosphorylation BioWizard is illustrated in the following tutorial:

Viewlet tutorial for the phosphorylation biowizard

[Note: To view the animated tutorials you need a Macromedia Flash Player. If your browser does not have a flash player plugin you can download it from the Macromedia web site]